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This Behind the Scenes article was provided to LiveScience in partnership with the National Science Foundation .
Basiru Leigh peer into a microscope in the lab of Oliver Hobert of Columbia University in NY . Leigh is examine mutant ground worm from a species known asC. elegans .
The nematode C.elegans under the microscope (imaged using bright light).
" There ! You see ? " he proclaim . " They are moving abnormally — in a circle instead of a U - embodiment . " This uncoordinated bm is a symptom of the worms ' motor neuron disease ; Leigh is trying to find the causal agent . His hypothesis : two transcription factors , proteins that regulate the formulation of other genes , play a role in the mutant worms ' abnormal movement .
Some of the most vexing and horrific human diseases , such as Spinal Muscular Atrophy and Amyotrophic Lateral Sclerosis , areneurological disordersthat result from the devolution and death of motor neurons , the cells that moderate muscle bodily process like speaking , walk , ventilation and swallowing .
Understanding the stem causes of these diseases would be one benefit of the research taking blank space in Hobert ’s lab at Columbia . Under Hobert ’s guidance , post - docs and alumnus students — and a smattering of younger students like Leigh — are examine to compute out how neurons develop and mark inC. elegans , a microscopic worm whose nervous scheme is correspondent in many ways to that of human beings .
Leigh has the chance to crop in Hobert ’s lab thanks to Harlem Children Society , an NSF - funded program produce ten long time ago by Sat Bhattacharya , a molecular geneticist at Memorial Sloan Kettering .
The end of the non - profit is to encourage low - income and nonage high schooltime students ( 95 percent minority ; more than 58 percent female ) to prosecute careers in scientific discipline , technology , technology , and maths ( STEM ) and ally disciplines through a unequalled , men - on program that includes pay enquiry internships at leading institutions and weekly workshops and seminar .
Harlem Children Society began in New York City , but has expanded and customized its political platform to meet local needs at rural and urban sites across the United States and the globe .
Leigh begin with Harlem Children Society five year ago , when he was 15 years old and entering his soph twelvemonth at the Bronx High School for Medical Science . He credit the course of study with his success at City College , where he just fill in his second year with a bivalent major in biology and computer science .
This summer , Leigh is one of two Harlem Children Society students working in Hobert ’s science lab . Leigh is working under the direct mentorship of Paschalis Kratsios , a post - doc whose inquiry focuses on the role of several written text factors in motor neuron ontogenesis and survival . recording gene are critical because of their role regularise the saying of so many other genes . An understanding of how they figure out could reserve scientists to control the process of neuron development and at long last find treatments for neurologic diseases likeParkinson’s , Alzheimer’sandAmyotrophic Lateral Sclerosis .
Kratsios has designed a simple but important project that Leigh can make out during the 12 - calendar week duration of his mentorship : a subject area of transcription factors Vab-15 and Zag-1 and their role in coordinate the movement ofC. elegans .
C. elegansis a particularly utile organism forneurological researchbecause it has a simple anxious system ( a total of 312 neurons ) and a three - day developmental cps . It also has transparent hide , which is helpful when examining motor nerve cell under a microscope .
Leigh began his research looking at the role of Transcription Factor Zag-1 using a canonical genetic experimentation . First , he multiply genetically normal worm , whose motor neurons were marked with green florescent proteins ( GFPs ) , with mutant worms neglect Zag-1 . Then Leigh secern out the issue demonstrating the same uncoordinated movements as their mutant parents . The issue ’s motor neurons were now all marked with GFPs and , using a florescent microscope , Leigh was able to bet them to find out if the uncoordinated progeny had fewer motor neurons than their normal parents .
Leigh ’s determination was surprising : the uncoordinated offspring did have all their motor neuron . Leigh was now able to conclude that the absence seizure of Zag-1 does not affect the survival of motor neuron . The uncoordinated movements of the mutant worms could rather ensue from some or all of the motor neurons not puzzle out right . But in exactly what way are they dysfunctional ? This interrogation is what Leigh hopes to reply in the next phase angle of his research .
To suffice it , Leigh will comport another genetic experiment . This time he will breed Zag-1 mutant worms with normal worms bear GFPs activate only when motor neuron resign their neurotransmitters . Leigh expect that when he examine the uncoordinated offspring of this new coupling , some of their motor neurons will not light up with GFP . This result would indicate that the absence of Zag-1 affects the neurotransmitter footpath inC. elegans’motor neurons .
Leigh say the experience of working with a mentor in a real lab is invaluable : " Dr. Kratsios explains everything in detail — I have learned so much more about genetic science here than in class . " And he is thrilled to have the chance to conduct his own research : " At my age , to get this opportunity to do my own inquiry … it ’s the beneficial . "
But for Leigh , who hope one day to be a neurosurgeon , the most exciting thing about the experience is participating in research that might one day chip in to treatments for disease like Parkinson ’s . " Muhammad Ali is one of my heroes , " Leigh enunciate , " so it ’s really cool to do something that could one solar day help him . "
